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Protein charge: the observed size could be influenced by the composition of amino acids (charged vs. Yeast Vps18 and Mon2 cDNA was amplified from yeast genomic DNA prepared from wild-type yeast with appropriate primers and subcloned into a yeast multicopy plasmid modified from YEplac181 ( 13 ) so that Vps18 or Mon2 was expressed under a constitutive ADH1 promoter (unpublished data). After setting aside all of your dice, if you are unable to claim a card, you gain a die of your choice for next turn. In addition, there are shared servers. Read the Bioinformatics Tool Guide for instructions on using this tool. I ask all sorts of support questions, some even relating to websites and not the hosting and each time they usually return with a helpful answer. Using coimmunoprecipitation and crosslinking analysis, Bishop and Woodman (2001) found that human VPS28 ( 601387 ) interacted with the coiled-coil C-terminal portion of TSG101. 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